Chris Pratt Mario (voice) Anya Taylor-Joy Princess Peach (voice) Charlie Day Luigi (voice) Jack Black Bowser (voice) Keegan-Michael Key Toad (voice) Seth Rogen Donkey Kong (voice) Fred Armisen Cranky Kong (voice) Khary Payton Penguin King (voice) Sebastian Maniscalco Spike (voice) Charles Martinet Mario's Dad / Giuseppe (voice) Kevin Michael Richardson Kamek (voice) Rino Romano Uncle Tony (voice) John DiMaggio Uncle Arthur (voice) Jessica DiCicco Mario's Mom / Yellow Toad / Plumbing Commercial Lady (voice) Eric Bauza General Toad (voice) Scott Menville Koopa General (voice) Carlos Alazraqui Additional Voices (voice) Ashly Burch Additional Voices (voice) Rachel Butera Additional Voices (voice) Cathy Cavadini Additional Voices (voice) Willow Geer Additional Voices (voice) Aaron Hendry Additional Voices (voice) Andy Hirsch Additional Voices (voice) Phil LaMarr Additional Voices (voice)